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With antibiotics yet to be invented, scientists in the 1890s pioneered the field of immunotherapy by injecting bacteria into horses and then extracting the antibody-rich serum to use as treatment for humans
Track results throughout maintenance phase
INTRODUCTION Cell culture The rat aortic A-10 VSMC line was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and was cultured in Dulbeccos modified Eagles medium (DMEM) high glucose (25 mmol/L, Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, Logan, UT, USA) and 1% penicillin/streptomycin (Gibco) in an incubator at 37C with a humidified atmosphere of 5% CO 2 in the air
Thinner needles create less tissue disruption
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