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Subsequently, daily oral gavage of metformin (200 mg/kg/day) commenced on the second day post-surgery and continued until euthanasia
Derivatized oestrogens were analysed in multiple-reaction monitoring scan mode (MRM) using positive ESI including the ion source parameters curtain gas (40 psi), ESI voltage (5,500 V), source temperature (500 C), gas 1 (70 psi) and gas 2 (50 psi)
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