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Description

Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template

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doi:10.1101/cshperspect.a006908 131

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IV delivery achieves therapeutic concentrations that oral glutathione cannot

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Anything that increases inflammation such as a lack of sleep, a diet high in inflammatory foods such as processed foods and sugar, smoking, or underlying infections, exposure to EMFs, an unhealthy gut, polluted indoor air or hidden mould

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Antioxidant Reactivation Glutathione works in tandem with other antioxidants to battle free radicals, but functions as more than just a good teammate

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