Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template
doi:10.1101/cshperspect.a006908 131
IV delivery achieves therapeutic concentrations that oral glutathione cannot
Anything that increases inflammation such as a lack of sleep, a diet high in inflammatory foods such as processed foods and sugar, smoking, or underlying infections, exposure to EMFs, an unhealthy gut, polluted indoor air or hidden mould
Antioxidant Reactivation Glutathione works in tandem with other antioxidants to battle free radicals, but functions as more than just a good teammate