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After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)

Exosome application: The regenerative solution is applied or injected, delivering growth factors directly to the hair follicles

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Chronic sleep deprivation has been associated with reduced GLP-1 response and increased appetite-stimulating hormones like ghrelin