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For the generation of mRFP-zDHHC5-KASH wild-type and DHHS-mutant, the sequences mRFP-zDHHC5 and mRFP-zDHHS5 were PCR-amplified from mRFP-fusions, cut with NheI/KpnI and used to exchange the GFP sequence cut with the same enzymes from GFP-KASH
Fifty microliters of stop solution was added to each well and the optical density at 450 nm determined using a microtiter plate reader (Liuyi, Beijing, China) within 15 min
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How does the Glutathione Injection work
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