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The dissociation constant ( K D ) values for Cu(II) in NTS of HSA (Asp-Ala-His) and of bovine serum albumin (Asp-Thr-His), have been determined by a variety of methods such as potentiometric titration, equilibrium dialysis, ultrafiltration, isothermal titration calorimetry, Cu(II) electrode and several spectroscopic methods, including EPR 27
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